Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (135,145)

Singleplex and Multiplex Bead Based Immunoassays (6,198)

ELISA Substrates and Reagents (2,230)

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Novus Biologicals™ Fish Thyroxine ELISA Kit (Colorimetric)

High sensitivity and specificity for detection of fish thyroxine.

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Invitrogen™ Cell Extraction Buffer

Suitable for use in ELISA and Western blotting

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Invitrogen™ Urea Nitrogen (BUN) Colorimetric Detection Kit

Assay Urea Nitrogen (BUN) levels in Serum, Plasma, Urine, Saliva and Tissue Culture Media.

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Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format	Colorimetric Detection Kit
Content And Storage	96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet. Store at 2–8°C.
For Use With (Equipment)	Microplate Reader
Gene ID (Entrez)	Non-Gene
Gene Symbol	Non-Gene
Target Kit Named	Urea Nitrogen (BUN)

Invitrogen™ Cell Lysis Buffer II

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

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Invitrogen™ Superoxide Dismutase (SOD) Colorimetric Activity Kit

Assay Superoxide Dismutase (SOD) Activity in Serum, Plasma, Cells, Tissues and Erythrocyte Lysates.

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Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format	Assay Activity Kit
Content And Storage	96-well plate(s), standard, detection reagent, assay buffer, substrate, and lot-specific technical data sheet. Store at –20°C.
For Use With (Equipment)	Microplate Reader
Target Kit Named	Superoxide Dismutase (SOD)

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R&D Systems™ Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit

Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

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Specifications

Target	CCL2/JE/MCP-1
Specificity	Natural and Recombinant Mouse MCP-1
Host Species	Mouse
Gene ID (Entrez)	6347
Gene Symbol	CCL2
Total Assay Time	4.5 hr.
Sample Volume	Cell Culture Supernates (50 uL),Tissue Lysates (50 uL),Tissue Culture Supernates (50 uL),Serum (50 uL),EDTA Plasma (50 uL),Heparin Plasma (50 uL)
Assay Sensitivity	0.666 pg/mL
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	7.81 - 500 pg/mL
For Use With (Application)	ELISA

Invitrogen™ Mouse SAA ELISA Kit

ELISA

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R&D Systems™ Human IFN-gamma Quantikine ELISA Kit

solid phase ELISA designed to measure human IFN-gamma levels in cell culture supernates, serum, and plasma.

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Specifications

Target	IFN-gamma
Specificity	Natural and Recombinant Human IFN-gamma
Target Species	Human
Gene ID (Entrez)	3458
Sample Type	Cell Culture Supernatant,Plasma (EDTA),Heparin Plasma,Serum
Synonym	IFG, IFI, IFNG, IFNgamma, IFN-gamma, Immune interferon, interferon gamma, interferon, gamma
Assay Sensitivity	5.69 pg/mL
Storage Requirements	Store the unopened product at 2°C to 8°C. Do not use past expiration date.
Assay Range	15.60 to 1000 pg/mL
For Use With (Application)	ELISA

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R&D Systems™ Human VEGF Quantikine ELISA Kit

Human VEGF Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

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Specifications

Specificity	Natural and Recombinant Human VEGF. This assay also recognizes Recombinant Human VEGF165b.
Target Species	Human
Conjugate	HRP
Gene ID (Entrez)	7422
Sample Type	Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum
Sample Volume	Cell Culture Supernates (200uL),Serum (100uL),EDTA Plasma (100uL),Heparin Plasma (100uL),Citrate Plasma (100uL)
Synonym	MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
Assay Sensitivity	5 pg/mL (Cell Culture Supernates), 9 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma)
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	15.60 to 1000 pg/mL (Cell Culture Supernates), 31.30 to 2000 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma)
For Use With (Application)	ELISA
Cross Reactivity	Cross-species reactivity observed with 1 or more species tested. <0.5% cross-reactivity observed with available related molecules.

Invitrogen™ Human TNF alpha ProQuantum Immunoassay Kit

ELISA

Pricing & Availability
Specifications

Immunoassay Kit Format	Proximity-based Immunoassay
Target Species	Human
Conjugate	FAM™
Incubation Time	1 hr.
Total Assay Time	2 hrs
Sample Volume	Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity	<0.01 pg/mL
Storage Requirements	Frozen at -20°C
Assay Range	0.0128 to 5000 pg/mL
For Use With (Application)	Protein Quantitation
Test Time	30 mins
Detection Method	Taqman™ Real-time PCR
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment)	qPCR Instruments
Product Type	ProQuantum
Gene ID (Entrez)	7124
Sample Type	Plasma,Serum,Supernatant
Label or Dye	FAM™

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R&D Systems™ Human IFN-gamma DuoSet ELISA

Human IFN-gamma DuoSet ELISA from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

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Specifications

Immunoassay Kit Format	96-well Plate
Target	Interferon gamma
Kit Contents	96 well Micro Plates,PBS,Plate Sealers,Reagent Diluents Concentrate 2,Stop Solution,Substrate Solution,Wash Buffer
Target Species	Human
Product Type	Solid Phase Sandwich ELISA
Gene ID (Entrez)	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Sample Type	Cell Culture Supernatant,Plasma (EDTA, Heparin),Serum
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	9.38 - 600pg/mL

Invitrogen™ Catalase Colorimetric Activity Kit

Assay Catalase Activity in Serum, Plasma, Cells, Tissues and Erythrocyte Lysates

Pricing & Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format	Assay Activity Kit
Content And Storage	96-well plate(s), standard, detection reagent, assay buffer, substrate, and lot-specific technical data sheet. Store at 2–8°C.
For Use With (Equipment)	Microplate Reader
Product Type	Catalase Colorimetric Activity Kit
Sample Type	Plasma,Serum
Target Kit Named	Catalase
Detection Method	Colorimetric

Invitrogen™ Human IL-1 beta ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	6.6%
Intraassay CV	4.4
Gene Symbol	IL-1,IL1-BETA,IL1F2
Total Assay Time	2 hrs 55 mins
Sample Volume	Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	1 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	3.9 to 250 pg/mL
For Use With (Application)	Colorimetric
Test Time	1 hrs 20 mins
Accession Number	P01584
Regulatory Status	RUO
Target	IL-1 beta
Kit Contents	Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision	1 pg/mL
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3553
Sample Type	Serum,Supernatant
Label or Dye	HRP

Invitrogen™ Mouse Estradiol Rapid ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Immunoassay Kit Format	Coated ELISA Kit
Target Species	Mouse
Conjugate	HRP
Interassay CV	<10%
Intraassay CV	<10%
Total Assay Time	1 hr 30 min
Assay Sensitivity	1.88 pg/mL
Storage Requirements	Refer to product documentation for component specific storage temperature.
Assay Range	3.13-200 pg/mL
Test Time	15 min
Regulatory Status	RUO
Target	Estradiol
Kit Contents	Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,HRP Conjugate,Standard & Sample Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer
Gene Alias	17-Beta-Estradiol; 17-beta-HSD17-Beta-Estradiol; 17-beta-HSD;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Sample Type	Plasma,Saliva,Serum,Urine
Label or Dye	HRP

Invitrogen™ Malondialdehyde (MDA) Colorimetric Assay Kit

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

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Novus Biologicals™ Fish Thyroxine ELISA Kit (Colorimetric) Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Invitrogen™ Human TNF alpha ProQuantum Immunoassay Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

R&D Systems™ Human IFN-gamma DuoSet ELISA GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Novus Biologicals™ Fish Thyroxine ELISA Kit (Colorimetric)

Invitrogen™ Human TNF alpha ProQuantum Immunoassay Kit

R&D Systems™ Human IFN-gamma DuoSet ELISA